Glycerol Bacteria Stocks[Cloning]

Long term storage of bacteria transformed with plasmid DNA.

Reagents:

Basic Protocol

1. Grow 1-2 ml of culture overnight or to saturation in LB medium plus appropriate antibiotic.

2. Mix 850 µl of the culture with 200 µl of 80% glycerol. The rest may be used for plasmid preparation.

3. Freeze quickly in liquid nitrogen or ethanol/dry ice.

4. Store at -80°C.

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