Brief Description
Long term storage of bacteria transformed with plasmid DNA.
Protocol Details
Reagents:
- 80% glycerol (sterile)
- (optional) liquid nitrogen or ethanol/dry ice
Basic Protocol
1. Grow 1-2 ml of culture overnight or to saturation in LB medium plus appropriate antibiotic.
2. Mix 850 µl of the culture with 200 µl of 80% glycerol. The rest may be used for plasmid preparation.
3. Freeze quickly in liquid nitrogen or ethanol/dry ice.
4. Store at -80°C.